This webpage is now under construction.

I will replace the old design by the new one, and update the content.

This will take some time.

This is my online manual about protein crystallization. At the moment you can find the following topics:

• The protein sample
  • Purity of the protein sample
    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
  • Sample homogeneity
  • Protein concentration and starting buffer
    • Protein assays
• Detergent
  • Detergent to protein ratio
• Crystallization methods
  • Vapor diffusion
  • Equilibrium dialysis
• Screening for crystals
  • Initial screen
  • Adjusted screen
  • Optimization screen
  • Additives
• Description of what you see in the drop
  • Examination of the crystallization experiments
  • Crystallization drop phenomena and interpretation
• References

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© 10-08-2018 Johan Zeelen