X-tal protocols: X-tal fact1

X-tal fact1

Date: .. - .. - .. Temperature: .... °C.  Experiment number:..........
Experiment name:...........................................................
Protein : .................................................................
[protein]      : ........ mg/ml
protein volume : ........ ul
Mother liquor  : ........ ul
Buffer ....................................................................

buffer pH precipitant1 precipitant2 result

100 mM

1A1 8.2 1.75 M (NH₄)₂HPO₄ ..............

1A2 Ada 6.5 2.0 M (NH₄)₂SO₄ 100 mM MgSO₄ ..............

1A3 Citrate 5.5 2.0 M (NH₄)₂SO₄ 200 mM NaCl ..............

1A4 Ches 9.5 2.0 M (NH₄)₂SO₄ 5 % MPD ..............

1A5 Tea 7.5 2.0 M (NH₄)₂SO₄ 2 % PEG 400 ..............

1A6 Ada 6.5 2.0 M (NH₄)₂SO₄ 10 % Ethanol ..............

1B1 Acetate 4.5 2.5 M (NH₄)₂SO₄ 200 mM Li₂SO₄ ..............

1B2 Tris 8.5 3.0 M (NH₄)₂SO₄ ..............

1B3 Tea 7.5 30 % t-butanol ..............

1B4 Ada 6.5 20 % Ethanol 200 mM KCl ..............

1B5 Citrate 5.5 30 % Ethanol ..............

1B6 Acetate 4.5 30 % Ethanol 10 % PEG 6000 ..............

1C1 Tris 8.5 40 % Ethanol 200 mM MgCl₂ ..............

1C2 Tea 7.5 10 % Isopropanol 20 % PEG 400 ..............

1C3 Acetate 4.5 20 % Isopropanol 200 mM KCl ..............

1C4 Citrate 5.5 20 % Isopropanol 2 % PEG 1500 ..............

1C5 Ches 9.5 30 % Isopropanol 200 mM MgSO4 ..............

1C6 5.5 1.5 M K₂ NaPO₄ ..............

1D1 Tea 7.5 1.5 M KNaTartrate ..............

1D2 Acetate 4.5 1.0 M Li₂SO₄ ..............

1D3 Tris 8.5 1.0 M Li₂SO₄ 1.0 M (NH₄)₂SO₄ ..............

1D4 Tea 7.5 1.5 M Li₂SO₄ ..............

1D5 Citrate 5.5 1.5 M MgSO₄ ..............

1D6 Tris 8.5 2.0 M MgSO₄ ..............

2A1 Ches 9.5 20 % MPD 200 mM MgCl₂ ..............

2A2 Citrate 5.5 30 % MPD ..............

2A3 Tris 8.5 30 % MPD 2.5 % t-butanol ..............

2A4 Acetate 4.5 40 % MPD ..............

2A5 Tea 7.5 40 % MPD 200 mM NaCl ..............

2A6 Ches 9.5 1.0 M Na Citrate ..............

2B1 Tea 7.5 1.5 M Na Citrate ..............

2B2 Tris 8.5 2.0 M NaCl ..............

2B3 Tea 7.5 2.0 M NaCl 10 % PEG 400 ..............

2B4 Acetate 4.5 3.0 M NaCl ..............

2B5 Acetate 4.5 2.0 M NaFormate ..............

2B6 Citrate 5.5 20 % PEG400 200 mM KCl ..............

2C1 Tea 7.5 30 % PEG 400 200 mM MgCl₂ ..............

2C2 Tris 8.5 40 % PEG 400 ..............

2C3 Tris 8.5 15 % PEG 1500 5 % MPD ..............

2C4 Citrate 5.5 20 % PEG 1500 ..............

2C5 Ada 6.5 20 % PEG 1500 200 mM KCl ..............

2C6 Ches 9.5 25 % PEG 1500 200 mM MgSO₄ ..............

2D1 Tea 7.5 30 % PEG 1500 200 mM (NH₄)₂SO₄ ..............

2D2 Acetate 4.5 20 % PEG 6000 1.0 M NaCL ..............

2D3 Citrate 5.5 20 % PEG 6000 2.5% t-butanol ..............

2D4 Tea 7.5 25 % PEG 6000 ..............

2D5 Ada 6.5 25 % PEG 6000 200 mM Li₂SO₄ ..............

2D6 Acetate 4.5 30 % PEG 6000 ..............

	buffer	pH	precipitant1	precipitant2	result
	100 mM
1A1		8.2	1.75 M (NH₄)₂HPO₄		..............
1A2	Ada	6.5	2.0 M (NH₄)₂SO₄	100 mM MgSO₄	..............
1A3	Citrate	5.5	2.0 M (NH₄)₂SO₄	200 mM NaCl	..............
1A4	Ches	9.5	2.0 M (NH₄)₂SO₄	5 % MPD	..............
1A5	Tea	7.5	2.0 M (NH₄)₂SO₄	2 % PEG 400	..............
1A6	Ada	6.5	2.0 M (NH₄)₂SO₄	10 % Ethanol	..............
1B1	Acetate	4.5	2.5 M (NH₄)₂SO₄	200 mM Li₂SO₄	..............
1B2	Tris	8.5	3.0 M (NH₄)₂SO₄		..............
1B3	Tea	7.5	30 % t-butanol		..............
1B4	Ada	6.5	20 % Ethanol	200 mM KCl	..............
1B5	Citrate	5.5	30 % Ethanol		..............
1B6	Acetate	4.5	30 % Ethanol	10 % PEG 6000	..............
1C1	Tris	8.5	40 % Ethanol	200 mM MgCl₂	..............
1C2	Tea	7.5	10 % Isopropanol	20 % PEG 400	..............
1C3	Acetate	4.5	20 % Isopropanol	200 mM KCl	..............
1C4	Citrate	5.5	20 % Isopropanol	2 % PEG 1500	..............
1C5	Ches	9.5	30 % Isopropanol	200 mM MgSO4	..............
1C6		5.5	1.5 M K₂ NaPO₄		..............
1D1	Tea	7.5	1.5 M KNaTartrate		..............
1D2	Acetate	4.5	1.0 M Li₂SO₄		..............
1D3	Tris	8.5	1.0 M Li₂SO₄	1.0 M (NH₄)₂SO₄	..............
1D4	Tea	7.5	1.5 M Li₂SO₄		..............
1D5	Citrate	5.5	1.5 M MgSO₄		..............
1D6	Tris	8.5	2.0 M MgSO₄		..............
2A1	Ches	9.5	20 % MPD	200 mM MgCl₂	..............
2A2	Citrate	5.5	30 % MPD		..............
2A3	Tris	8.5	30 % MPD	2.5 % t-butanol	..............
2A4	Acetate	4.5	40 % MPD		..............
2A5	Tea	7.5	40 % MPD	200 mM NaCl	..............
2A6	Ches	9.5	1.0 M Na Citrate		..............
2B1	Tea	7.5	1.5 M Na Citrate		..............
2B2	Tris	8.5	2.0 M NaCl		..............
2B3	Tea	7.5	2.0 M NaCl	10 % PEG 400	..............
2B4	Acetate	4.5	3.0 M NaCl		..............
2B5	Acetate	4.5	2.0 M NaFormate		..............
2B6	Citrate	5.5	20 % PEG400	200 mM KCl	..............
2C1	Tea	7.5	30 % PEG 400	200 mM MgCl₂	..............
2C2	Tris	8.5	40 % PEG 400		..............
2C3	Tris	8.5	15 % PEG 1500	5 % MPD	..............
2C4	Citrate	5.5	20 % PEG 1500		..............
2C5	Ada	6.5	20 % PEG 1500	200 mM KCl	..............
2C6	Ches	9.5	25 % PEG 1500	200 mM MgSO₄	..............
2D1	Tea	7.5	30 % PEG 1500	200 mM (NH₄)₂SO₄	..............
2D2	Acetate	4.5	20 % PEG 6000	1.0 M NaCL	..............
2D3	Citrate	5.5	20 % PEG 6000	2.5% t-butanol	..............
2D4	Tea	7.5	25 % PEG 6000		..............
2D5	Ada	6.5	25 % PEG 6000	200 mM Li₂SO₄	..............
2D6	Acetate	4.5	30 % PEG 6000		..............