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X-tal protocols

X-tal fact1

Protein Detergent Methods Euipment Screen Drops Reference

Date: .. - .. - .. Temperature: .... °C.  Experiment number:..........
Experiment name:...........................................................
Protein : .................................................................
[protein]      : ........ mg/ml
protein volume : ........ ul
Mother liquor  : ........ ul
Buffer ....................................................................

bufferpHprecipitant1precipitant2result
100 mM
1A18.2 1.75 M (NH4)2HPO4..............
1A2Ada6.52.0 M (NH4)2SO4100 mM MgSO4..............
1A3Citrate5.52.0 M (NH4)2SO4200 mM NaCl..............
1A4Ches9.52.0 M (NH4)2SO45 % MPD..............
1A5Tea7.52.0 M (NH4)2SO42 % PEG 400..............
1A6Ada6.52.0 M (NH4)2SO410 % Ethanol..............
1B1Acetate4.52.5 M (NH4)2SO4200 mM Li2SO4..............
1B2Tris8.53.0 M (NH4)2SO4..............
1B3Tea7.530 % t-butanol ..............
1B4Ada6.520 % Ethanol 200 mM KCl..............
1B5Citrate5.530 % Ethanol ..............
1B6Acetate4.530 % Ethanol 10 % PEG 6000..............
1C1Tris8.540 % Ethanol 200 mM MgCl2..............
1C2Tea7.510 % Isopropanol 20 % PEG 400..............
1C3Acetate4.520 % Isopropanol 200 mM KCl..............
1C4Citrate5.520 % Isopropanol 2 % PEG 1500..............
1C5Ches9.530 % Isopropanol 200 mM MgSO4..............
1C65.51.5 M K2 NaPO4..............
1D1Tea7.51.5 M KNaTartrate ..............
1D2Acetate4.51.0 M Li2SO4..............
1D3Tris8.51.0 M Li2SO41.0 M (NH4)2SO4..............
1D4Tea7.51.5 M Li2SO4..............
1D5Citrate5.51.5 M MgSO4..............
1D6Tris8.52.0 M MgSO4..............
2A1Ches9.520 % MPD200 mM MgCl2..............
2A2Citrate5.530 % MPD ..............
2A3Tris8.530 % MPD 2.5 % t-butanol..............
2A4Acetate4.540 % MPD ..............
2A5Tea7.540 % MPD 200 mM NaCl..............
2A6Ches9.51.0 M Na Citrate ..............
2B1Tea7.51.5 M Na Citrate ..............
2B2Tris8.52.0 M NaCl ..............
2B3Tea7.52.0 M NaCl 10 % PEG 400..............
2B4Acetate4.53.0 M NaCl ..............
2B5Acetate4.52.0 M NaFormate ..............
2B6Citrate5.520 % PEG 400 200 mM KCl..............
2C1Tea7.530 % PEG 400200 mM MgCl2..............
2C2Tris8.540 % PEG 400 ..............
2C3Tris8.515 % PEG 1500 5 % MPD ..............
2C4Citrate5.520 % PEG 1500 ..............
2C5Ada6.520 % PEG 1500 200 mM KCl..............
2C6Ches9.525 % PEG 1500 200 mM MgSO4..............
2D1Tea7.530 % PEG 1500200 mM (NH4)2SO4..............
2D2Acetate4.520 % PEG 6000 1.0 M NaCL ..............
2D3Citrate5.520 % PEG 6000 2.5% t-butanol ..............
2D4Tea7.525 % PEG 6000 ..............
2D5Ada6.525 % PEG 6000200 mM Li2SO4..............
2D6Acetate4.530 % PEG 6000 ..............

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