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X-tal protocols

X-tal fact2

Protein Detergent Methods Euipment Screen Drops Reference
Date: .. - .. - .. Temperature: .... °C.  Experiment number:..........
Experiment name:...........................................................
Protein : .................................................................
[protein]      : ........ mg/ml
protein volume : ........ ul
Mother liquor  : ........ ul
Buffer ....................................................................
pHadditiveprecipitant1precipitant2 result
1A15.510 % DIOXANE2.0 M (NH4)2SO4..............
1B17.51 % DIOXANE20 % PEG 6000 ..............
1C19.010 % DIOXANE20 % Isopropanol ..............
1D16.520 % DIOXANE ..............
1A28.010 % DMSO2.45 M (NH4)2SO4..............
1B29.010 % DMSO10 % MME-PEG 500010 % MPD ..............
1C27.010 % DMSO20 % MPD ..............
1D26.030 % DMSO10 % Jeffamine ..............
1A38.53.0 M CsCl 1.0 M (NH4)2SO4 ..............
1B34.5200 mM CsCl 15 % MME-PEG 5000 ..............
1C37.0200 mM CsCl 2.0 M Hexane-1,6-diol ..............
1D36.53.0 M CsCl ..............
1A45.52.5 % 1-octanol 1.5 M (NH4)2SO4..............
1B47.52.5 % 1-octanol 15 % PPG P-425 ..............
1C46.02.5 % 1-octanol 20 % Isopropanol ..............
1D48.02.5 % 1-octanol 1.0 M KCl ..............
1A58.510 % Glycerol 2.45 M (NH4)2SO4..............
1B56.020 % Glycerol 10 % PEG 6000 ..............
1C57.010 % Glycerol 20 % MPD ..............
1D55.510 % Glycerol 2.0 M NaCl ..............
1A68.00.8 % ß-octyl glucoside 2.0 M (NH4)2SO420 % Ethyleneglycol..............
1B67.50.1 % ß-octyl glucoside 15 % PEG 6000 ..............
1C66.50.1 % ß-octyl glucoside 20 % MPD ..............
1D64.50.5 % ß-octyl glucoside 3.0 M NaCL ..............

2A15.50.01 % NP-40 1.5 M (NH4)2SO4..............
2B17.00.01 % NP-40 20 % MME-PEG 5000 ..............
2C14.50.01 % NP-40 20 % MPD1.0 M (NH4)2SO4 ..............
2D18.50.01 % NP-40 2.0 M LiCl2 ..............
2A26.0200 mM Cobalt Sulfate 1.0 M (NH4)2SO4..............
2B24.550 mM Cobalt Sulfate 15 % Jeffamine M-600 ..............
2C27.5200 mM Cobalt Sulfate 1.5 M Hexane-1,6-diol ..............
2D27.01.5 M Cobalt Sulphate ..............
2A38.01.0 M NaCl 2.0 M (NH4)2SO4 ..............
2B36.52.0 M NaCl 10 % PEG 6000 10 % MME-PEG 5000 ..............
2C37.5200 mM NaCl 1.5 M Hexane-1,6-diol ..............
2D36.02.0 M NaCl 30 % Ethyleneglycol ..............
2A48.050 mM MgCl2, 50 mM KCl1.5 M (NH4)2SO4 10 % glycerol ..............
2B44.550 mM MgCl2, 50 mM KCl20 % Jeffamine M-600 ..............
2C46.550 mM MgCl2, 50 mM KCl30 % MPD ..............
2D47.01.0 M MgCl2 1.0 M KCl ..............
2A57.02.5 M LiCl2 200 mM (NH4)2SO4 ..............
2B55.51.0 M LiCl2 20 % Ethyleneglycol ..............
2C59.01.0 M LiCl2 20 % PPG P-425 ..............
2D58.51.0 M LiCl2 1.0 M NaCl ..............
2A65.51.0 M KCl 1.0 M (NH4)2SO4..............
2B68.0200 mM KCl 20 % PEG 6000 20 % Ethyleneglycol ..............
2C67.0200 mM KCl 20 % Jeffamine M-600 ..............
2D66.02.0 M KCl ..............

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