Back
X-tal protocols

Pipetting X-tal fact1

Protein Detergent Methods Euipment Screen Drops Reference
The pipetting scheme is based on two trays of 24 wells each. Pipette the indicated volumes in microliter in the corresponding wells. The total volume in the well is 1 ml. Work carefully to prevent mistakes.
Tray 1Tray 2
1. H20
125289289229289229
7543600500600350
400400500650520250
1503445967300100
1. H20
40030027510060344
67500300300400300
200100425400200195
93200375275164150
2. 50 % MPD
---100--
------
------
------
2. 50 % MPD
400600600800800-
------
--100---
------
3. 50 % PEG 400
----40-
------
-400----
------
3. 50 % PEG 400
------
--200--400
600800----
------
4. 40 % PEG 1500
------
------
---50--
------
4. 40 % PEG 1500
------
------
--375500500625
750-----
5. 40 % PEG 6000
------
-----250
------
------
5. 40 % PEG 6000
------
------
------
-500500625625750
6. 1.0 M Acetic acid/NaOH
------
100----100
--100---
-100----
6. 1.0 M Acetic acid/NaOH
---100--
---100100-
------
-100---100
7. 1.0 M Citric Acid/NaOH
--100---
----100-
---100--
----100-
7. 1.0 M Citric Acid/NaOH
-100----
-----100
---100--
--100---
8. 1.0 M Ada/NaOH
-100---100
---100--
------
------
8. 1.0 M Ada/NaOH
------
------
----100-
----100-
9. 1.0 M Tea/HCl
----100-
--100---
-100----
100--100--
9. 1.0 M Tea/HCl
----100-
100-100---
100-----
100--100--
10. 1.0 M Tris/HCl
------
-100----
100-----
--100--100
10. 1.0 M Tris/HCl
--100---
-100----
-100100---
------
11. 1.0 M Ches/NaOH
---100--
------
----100-
------
11. 1.0 M Ches/NaOH
100----100
------
-----100
------
12. 1.0 M KCl
------
---200--
--200---
------
12. 1.0 M KCl
------
-----200
----200-
------
13. 2.0 M (NH4)2HPO4
875-----
------
------
------
13. 2.0 M (NH4)2HPO4
------
------
------
------
14. 3.5 M (NH4)2SO4
-571571571571571
714857----
------
--286---
14. 3.5 M (NH4)2SO4
------
------
------
57-----
15. 1.8 M Li2SO4
------
111-----
------
-556556833--
15. 1.8 M Li2SO4
------
------
------
----111-
16. 2.0 M MgCl2
------
------
100-----
------
16. 2.0 M MgCl2
100-----
------
100-----
------
17. 2.5 M MgSO4
-40----
------
----80-
----600800
17. 2.5 M MgSO4
------
------
-----80
------
18. 2.0 M KH2PO4/NaOH
------
------
-----750
------
18. 2.0 M KH2PO4/NaOH
------
------
------
------
19. 2.0 M KNaTartrate
------
------
------
750-----
19. 2.0 M KNaTartrate
------
------
------
------
20. 1.8 M Na3Citrate
------
------
------
------
20. 1.8 M Na3Citrate
-----556
833-----
------
------
21. 5.0 M NaCl
--40---
------
------
------
21. 5.0 M NaCl
----40-
-400400600--
------
-200----
22. 4.0 M Naformate
------
------
------
------
22. 4.0 M Naformate
------
----500-
------
------
23. 100 % tert-butanol
------
--300---
------
------
23. 100 % tert-butanol
--25---
------
------
--25---
24. 100 % Ethanol
-----100
---200300300
400-----
------
24. 100 % Ethanol
------
------
------
------
25. 100 % Isopropanol
------
------
-100200200300-
------
25. 100 % Isopropanol
------
------
------
------

Top Back © 18 Febr 2004 Johan Zeelen