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X-tal protocols

Pipetting Xtal fact2

Protein Detergent Methods Euipment Screen Drops Reference
The pipetting scheme is based on two trays of 24 wells each. Pipette the indicated volumes in microliter in the corresponding wells. The total volume in the well is 1 ml. Work carefully to prevent mistakes.
Tray 1Tray 2
1. H20
129-113447-49
380250492575250515
500300367675300490
500100400625300250
1. H20
371513129233218363
300575-462450150
113425485262250450
300150200150450400
2. 40% PEG 6000
------
500---250375
------
------
2. 40% PEG 6000
------
--250--500
------
------
3. 40% MME-PEG 5000
------
-250-375---
------
------
3. 40% MME-PEG 5000
------
500-250---
------
------
4. 50% Jeffamine/HCl pH=7.5
------
------
------
-200----
4. 50% Jeffamine/HCl pH=7.5
------
-300-400--
-----400
------
5. 50% PPG P-425
------
---300--
------
------
5. 50% PPG P-425
------
------
----400-
------
6. 50% MPD
------
-200----
-400--400400
------
6. 50% MPD
------
------
400--600--
------
7. 100 % Isopropanol
------
------
200--200--
------
7. 100 % Isopropanol
------
------
------
------
8. 100 % EthyleneGlycol
-----200
------
------
------
8. 100 % EthyleneGlycol
------
----200200
------
--300---
9. 50 % Glycerol
----200-
----400-
----200-
----200-
9. 50 % Glycerol
---200--
------
------
------
10. Citric acid/btppH=4.0
702010701020
30 090306030
04040604050
506050207090
10. Citric acid/btppH=4.0
706020104070
409050807020
90303050 040
104060401060
11. NaCitrate/btp pH=9.0
308090309080
7010010704070
100 6060406050
50 4050803010
11. NaCitrate/btp pH=9.0
304080906030
60105020 3080
1070705010060
90604060 9040
12. 3.5 M (NH4)2SO4
571700287428700571
------
------
------
12. 3.5 M (NH4)2SO4
42928757142957287
------
287-----
------
13. 6.0 M CsCl
--500---
-- 33---
-- 33---
--500---
13. 6.0 M CsCl
------
------
------
------
14. 2.0 M CoSO4
------
------
------
------
14. 2.0 M CoSO4
-100----
- 25----
-100----
-750----
15. 4.0 M KCl
------
------
------
---250--
15. 4.0 M KCl
---13-250
--- 13- 50
--- 13- 50
---250-500
16. 4.0 M LiCl2
------
------
------
------
16. 4.0 M LiCl2
----625-
----250-
----250-
500---250-
17. 5.0 M NaCl
------
------
------
----400600
17. 5.0 M NaCl
--200---
--400---
--40---
--400-200-
18. 2.0 M MgCl2
------
------
------
------
18. 2.0 M MgCl2
---25--
---25--
---25--
---500--
19. 50 % Dioxane
200-----
20-----
200-----
400-----
19. 50 % Dioxane
------
------
------
------
20. 50 % DMSO
-200----
-200----
-200----
-600----
20. 50 % DMSO
------
------
------
------
21. 100 % 1-Octanol
---25--
---25--
---25--
---25--
21. 100 % 1-Octanol
------
------
------
------
22. 4.0 M 1,6 Hexane-diol
------
------
--500---
------
22. 4.0 M 1,6 Hexane-diol
------
------
-375375---
------
23. 10 % ß-OG
-----80
-----10
-----10
-----50
23. 10 % ß-OG
------
------
------
------
24. 0.1 % NP-40
------
------
------
------
24. 0.1 % NP-40
100-----
100-----
100-----
100-----

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